Product Manual (.pdf)

Description

This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative measurement of human myeloperoxidase (MPO) levels in serum and EDTA-plasma samples. MPO serves as a sensitive predictor for cardiovascular disease.

For research use only. Not for use in diagnostics procedures.

Background

Myeloperoxidase (MPO) is a specific polymorphonuclear enzyme that is most abundantly expressed in neutrophil granulocytes. It functions in the oxygen-dependent killing of microorganisms and is released from primary granules of neutrophils during acute inflammation. MPO is encoded by the MPO gene, which is about 11kb and composed of 11 introns and 12 exons, located in chromosome 17 (q12-24). The mature 150 kDa MPO protein is a dimer consisting of two 15kDA light chains and two heavy chains in variable degrees of glycosylation.

MPO is related both to inflammation and oxidative stress. It is a sensitive predictor for myocardial infarction in patients presenting chest pain. Studies ahve indicated that MPO is causally linked to atherosclerosis. Moreover, a combination test of MPO and CRP (C-reactive protein) provides added benefit for risk prediction of cardiovascular mortality than by simply measuring CRP alone. This assay utilizes a specific monoclonal antibody to capture MPO in test samples to ensure that only myeloperoxidase is detected.

Specifications

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

KTR-890

Myeloperoxidase (MPO)

Human

Sandwich ELISA

96 tests

Colorimetric

0.65 ng /mL

0 - 512 ng/mL

1 hour, 50 minutes

serum, EDTA-plasma

100 µL

2-8 °C