Product Manual (.pdf)

Description

This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative measurement of human adrenocorticotropic hormone in EDTA-plasma. This kit is useful for detecting elevated and deficient ACTH levels.

For research use only. Not for use in diagnostics procedures.

Background

ACTH is a 39-residue polypeptide with a molecular weight of 4540 Da. It is secreted from corticotropes in the anterior lobe (adenohypophysis) of the pituitary gland in response to cortocotrop-releasing hormone (CRH) released by the hypothalamus. ACTH is synthesized from pre-pro-opiomelanocortin (pre-POMC) The removal of the signal peptide during translation produces the 241-amino acid polypeptide POMC, which undergoes a series of post-translational modifications, such as phorphorylation and glycosylation, before it is proteolytically cleaved by endopeptidases to yield various polypeptide fragments with varying physiological activity.

ACTH is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress. It stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal gland. ACTH acts by binding to cell surface ACTH receptors, which are located primarily on adrenocortical cells of the adrenal cortex.

Specifications

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

KTR-882

Adrenocorticotropic Hormone (ACTH)

Human

Sandwich ELISA

96 tests

Colorimetric

< 1 pg /mL

0 - 416 ng/mL

2 hour, 20 minutes

EDTA-plasma

200 µL

2-8 °C