Product Manual (.pdf)

WHY measure Auto-antibodies to FcεR1α and hIgE?

Description

This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human anti-human IgE antibody levels in human serum or plasma samples. The test may be useful for detecting patients who have developed antibodies (mainly IgG) to their own IgE. 

For research use only. Not for use in diagnostic procedures.

Background

The presence of anti-IgE antibodies in patient serum or plasma has been associated with chronic urticaria, which is a common skin disorder affecting 0.5% to 1.8% of the general population. It is characterized by repeated occurrence of short-lived cutaneous wheals accompanied by redness and itching. This anti-IgE antibody ELISA is a ready-to-use test kit with well-breakable microtiter plate and simple test procedures.

Related Kits

KTR-802 Serolisa™ Anti-IgE Receptor (FcɛR1α) Autoantibody ELISA

Specifications

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

KTR-803

Human anti-IgE antibody

Human

Sandwich ELISA

96 tests

Colorimetric

0.4804 ng/mL

0 - 450 ng/mL

2 hours 20 min

Serum or plasma

100 µL

2-8 °C

Related Literature

1. Fraser, K., Robertson, L. Chronic Urticaria and Autoimmunity. Skin Therapy Letter. 2014;18(7). 
2. Greenberger, P. Chronic urticaria: new management options. World Allergy Organization Journal. 2014;7(70).
3. Konstantinou GN, Asero R, Maurer M, Sabroe RA, Schmid-Grendelmeier P, Grattan CE. EAACI/GA(2)LEN task force consensus report: The autologous serum skin test in urticaria. Allergy. 2009;64(9):1256-1268. doi: 10.1111/j.1398-9995.2009.02132.x.
4. Campos, V, Yassumoto, L, Filho, O, Antunes, R, Calamita, Z. Chronic spontaneous urticaria: cutaneous reaction and laboratory aspects. J Bras Patol Med Lab; 52(2), 84-90. 10.5935/1676-2444.20160020