Human Fetuin-A ELISA Kit

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Description

This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative determination of human fetuin-A, also known as alpha-2-HS glycoprotein (AHSG), in serum, plasma, cell culture supernatant, tissue extraction, and urine. The measurement of fetuin-A in serum or plasma aids in the diagnosis of some cancers and genetically inherited deficiencies of this serum protein. 

For research use only. Not for use in diagnostics procedures.

Background

Fetuin-A, also known as alpha-2-HS glycoprotein, is a 59 kDa glycoprotein that consists of two amino-terminal cystatin domains and a smaller carboxy-terminal domain. It is synthesized by the liver and secreted into blood stream, where its concentration in adult mammals ranges from 0.5 – 1.0 g/L. Fetuin-A occurs with high concentration in serum during fetal life and is involved in protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. It accumulates in bones and teeth as a major fraction of noncollagenous bone proteins. Biological studies have demonstrated that fetuin-A is the major calcification inhibitor found in circulation, where it interferes with calcium salt precipitation.

Recent studies have indicated that fetuin-A levels drop in uremic patients on hemodialysis in comparison to normal healthy controls. The low fetuin-A level in patients with chronic kidney failure strongly associates with higher cardiovascular mortality. On the other hand, it has been demonstrated that a higher than normal serum fetuin-A in an older population is associated with diabetes, which is independent from other markers of insulin resistance. Furthermore, a higher fetuin-A level may be an independent risk marker of patients with cardiovascular diseases.

Related Products

KTR-900 High Sensitive Human Fetuin-A ELISA Kit

 

Specifications

 

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

 

KT-800

Fetuin-A / alpha-2-HS glyocoprotein / AHSG

Human

Sandwich ELISA

96 tests

Colorimetric

5 ng/mL

0 - 370 ng/mL

2 hours, 50 minutes

Serum, plasma, urine, cell culture supernatant, tissue extraction

10 µL

2-8 °C

Selected Literature

  1. Lim P, Collet JP, Moutereau S, Guigui N, Mitchell-Heggs L, Loric S, Bernard M, Benhamed S, Montalescot G, Randé JL, Guéret P. Fetuin-A is an independent predictor of death after ST-elevation myocardial infarction. Clin Chem. 2007 Oct;53(10):1835-40. Epub 2007 Aug 16
     
  2. Stenvinkel P, Wang K, Qureshi AR, Axelsson J, Pecoits-Filho R, Gao P, Barany P, Lindholm B, Jogestrand T, Heimbürger O, Holmes C, Schalling M, Nordfors L. Low fetuin-A levels are associated with cardiovascular death: Impact of variations in the gene encoding fetuin. Kidney Int. 2005 Jun;67(6):2383-92.
     
  3. Sato H, Kazama JJ, Wada Y, Kuroda T, Narita I, Gejyo F, Gao P, Yamashita H. Decreased levels of circulating alpha2-Heremans-Schmid glycoprotein/Fetuin-A (AHSG) in patients with rheumatoid arthritis. Intern Med. 2007;46(20):1685-91. Epub 2007 Oct 15.

For research use only. Not for use in diagnostics procedures.