Product Manual (.pdf)

Description

This kit is intended for the extraction of glucagon-like peptide 1 (GLP-1) from serum of plasma samples. Following extraction, sample GLP-1 concentration may be measured by using GLP-1 immunoassays.

For research use only. Not for use in diagnostics procedures.

Background

Many immunoassays have been developed for measuring serum GLP-1 levels. However, studies have found that the direct measurement of GLP-1 concentration in serum generates unreliable test results, which are not clinically meaningful and do not correlate between multiple assays. Previous studies have suspected that there is an unknown matrix effect for measuring GLP-1 in serum sample by using immunoassays. Dai, et al. reported that solid phase extraction of serum sample before GLP-1 immunoassays eliminates the matrix effect and gives excellent GLP-1 test results, which include 1) clinical, meaningful fast/fed GLP-1 serum concentration from normal donors and 2) excellent correlation among three different immunoassays for GLP-1 in serum. Therefore, it is essential to perform a sample extraction before assaying GLP-1.

There are several extraction methods currently available. Most methods require expensive and specialized laboratory equipment, such as a vacuum centrifuge. This kit provides a well-validated solid phase GLP-1 sample extraction method by using a  reliable peptide extraction cartridge with excellent recovery and reproducibility. The procedure is easy to perform and well-suited for managing a large number of samples without special equipment.

Specifications

Catalog no.

Target:

Species

Method

Tests Per Kit

KT-910

Glucagon-like peptide 1 (GLP-1)

Human

Peptide extraction

50 tests