Product Manual (.pdf)

Description

This ELISA (enzyme-linked immunosorbent assay) kit is intended for the quantitative measurement of human myeloperoxidase (MPO) levels in stool and urine samples, which may serve as a sensitive predictor for inflammatory activities in the gastrointestinal tract.

This ELISA kit is for in-vitro diagnostic use.

Background

Myeloperoxidase (MPO) is a specific polymorphonuclear enzyme that is most abundantly expressed in neutrophil granulocytes. It functions in the oxygen-dependent killing of microorganisms and is released from primary granules of neutrophils during acute inflammation. MPO is encoded by the MPO gene, which is about 11kb and composed of 11 introns and 12 exons, located in chromosome 17 (q12-24). The mature 150 kDa MPO protein is a dimer consisting of two 15kDA light chains and two heavy chains in variable degrees of glycosylation.

MPO activity has been found to be linearly related to  neutrophil cell count. Since neutrophils play a predominant role in inflammatory and immune reactions in inflammatory bowel disease (IBD) and MPO has been observed both in the intestinal mucosa and  gut lavage, the determination of MPO in stool sample provides one of the most sensitive and promising biomarkers in predicting disease severity. This assay utilizes a specific monoclonal antibody to capture MPO in test samples in order to ensure that only myeloperoxidase is detected.

Related Kits

KT-890 Quantitative Serum/Plasma Myeloperoxidase ELISA Kit

KT-844 Fecal MPO Sample Collection Kit

Specifications

Catalog no.

Target:

Species

Method

Tests Per Kit

Detection

Sensitivity / LLOD

Dynamic Range

Total Incubation Time

Sample Type

Sample Volume

Storage Temperature

KT-880

Myeloperoxidase (MPO)

Human

Sandwich ELISA

96 tests

Colorimetric

0.15 µg /mL

0 - 256 µg/mL

2 hour, 35 minutes

Stool, urine

100 µL

2-8 °C